关键词: ChromoTek—单克隆抗体、重组单域抗体、荧光抗体、GFP或RFP连接蛋白
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GFP-Trap®

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GFP-Trap®_A

发布时间:2017/05/16 点击量:

    Effective pulldown for consistent results

Description: 
anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins

 

Specificity:

  • AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP K206A
  • CFP, eCFP, mCerulean
  • YFP, Citrine, eCitrine, eYFP, Venus, Ypet
  • BFP
     

Applications:

  • Immunoprecipitation/ Co-IP
  • Mass spectrometry
  • Enzyme activity measurements
  • RIP analysis


Bead properties: 
Bead size: ~ 90µm (cross-linked 4% agarose beads)
Storage buffer: 20% EtOH 
Binding capacity: 10µl GFP-Trap®_A slurry binds 4µg of recombinant protein

Storage instructions:
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.

Effective pulldown of GFP-fusion proteins for consistent results

  • No heavy & light antibody chains, short incubation (5-30 min)
  • Extraordinary binding, also under harsh conditions
  • Very high affinity (KD=1pM*) to bind even low abundant proteins
  • Reliable gold standard with more than 900 publications

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