Visualize the actin dynamics in real time in the nucleus with the nuclear Actin-Chromobody®. Upon transformation, your cells express the nuclear Actin-Chromobody® and transport the nuclear Actin-Chromobody into the nucleus. The nuclear cytoskeleton is visualized in real time by binding of the fluorescent Chromobody® to Actin.Actin-Chromobody®
Courtesy of Matthias PlessnerNIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody® (TagGFP2, green) and the Lamin-Chromobody® (TagRFP2, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody-probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum.
Trace actin dynamics in cell nuclei non-invasively
With the nuclear Actin-Chromobody® plasmid you obtain the sequence of the Alpaca antibody to Actin, also termed VHH-Actin or Actin-nanobody, fused to TagGFP (Evrogen) and a nuclear localization sequence (NLS). The transient binding does not influence cell viability or motility and thus offers you the unique possibility to observe the cytoskeleton in your cell nuclei.
The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:
Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (PubMed)