关键词: ChromoTek—单克隆抗体、重组单域抗体、荧光抗体、GFP或RFP连接蛋白
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Chromobodies®

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HeLa-CCC-TagRFP

发布时间:2017/05/16 点击量:

 

   HeLa-CCC-TagRFP

 

  Description

  HeLa-Cell Cycle Chromobody®-TagRFP

  HeLa cell line stably expressing Cell Cycle Chromobody® fused to the red fluorescent protein TagRFP

  Specificity

  VHH antibody fragment against cell cycle marker PCNA (proliferating cell nuclear antigen)Actin-Chromobody®

  Live cell imaging of HeLa-Cell Cycle Chromobody®-TagRFP

  

 

  For live cell imaging cells were seeded in ibidi µ-Slides. Time series was acquired on a spinning disk microscope (UltraVIEW VoX, PerkinElmer) equipped with a 63x/1.4 NA Plan-Apochromat oil immersion objective and a humidified, temperature- and CO2-controlled live cell chamber. TagRFP was excited with a 561 nm laser-line for typically between 100-500 ms/ image with laser intensity set to max. 10%. Confocal image z-stacks of living cells were recorded with a frame size of 1000x1000 pixels, a pixel size of 100 nm and a z-step size of 400 nm every 30 minutes. Images shown are maximum intensity projections of few mid-z-sections.