关键词: ChromoTek—单克隆抗体、重组单域抗体、荧光抗体、GFP或RFP连接蛋白
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F2H® Assay

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F2H®-Kit Basic

发布时间:2017/04/19 点击量:

  F2H®-Kit Basic allows effortless imaging of interactions between GFP- and RFP-tagged proteins. Study your proteins of interest and domains thereof in live mammalian cells. You just need your GFP-bait and RFP-prey plasmids ready for transfection.

  F2H® principle

  F2H® cells (BHK origin) carry a GFP-anchoring platform in the nucleus

  GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H® cells

  GFP-bait is recruited to the platform → bright green spot

  If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot

  If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence

  


  F2H® is easy

  You get the F2H® cell line

  You get the Platform Reagent

  You have your GFP-bait and RFP-prey plasmids

  Day 1: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H® cells

  Day 2: Image and quantify your results


 

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