关键词: ChromoTek—单克隆抗体、重组单域抗体、荧光抗体、GFP或RFP连接蛋白
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F2H® Assay

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F2H®-Kits p53-Mdm2/4

发布时间:2017/05/16 点击量:

  F2H®-Kits p53-Mdm2/4

  The Fluorescent Two-Hybrid (F2H®) Kits for p53-Mdm2 and for p53-Mdm4 respectively enable intracellular analysis of the interaction between the tumor suppressor p53 and its negative regulators Mdm2 or Mdm4.

  GFP-tagged human p53 is anchored at a specific location in the nucleus of F2H®-Cells, forming a bright fluorescent spot in the green channel.

  Interaction with the RFP-tagged human Mdm2 or the RFP-tagged human Mdm4 can be easily evaluated by conventional fluorescence microscopy as enrichment of red fluorescence at the location of the green spot.

  Compounds’ ability to disrupt the p53 and Mdm2 interaction or the p53 and Mdm4 interaction is determined based on the disappearance of the red spots from the anchored green spots.

F2H p53-Mdm4
  • Monitor protein dynamics upon treatments
  • Screen protein interaction inhibitors

Please inquire if you want to learn more about our F2H® Assay Kits.
















  Results

  F2H® allows direct side-by-side comparison of the compound's activity and kinetics in live cells.

  p53:Mdm2 + Nutlin

  

 

  p53:Mdm2 + sMTide02

  

 

  p53:Mdm4 + Nutlin

  

 

  p53:Mdm4 + sMTide02

  

 

  Incubations with 50 μm Nutlin-3 or stapled peptide sMTide-02 were carried out for 7 h during imaging. Fading of the red fluorescence from the spot but not from the rest of the nucleus indicates disruption of the interaction.